Soil metaproteomics – Comparative evaluation of protein extraction protocols
نویسندگان
چکیده
منابع مشابه
Metaproteomics: Evaluation of protein extraction from activated sludge.
Metaproteomic studies of full-scale activated sludge systems require reproducible protein extraction methods. A systematic evaluation of three different extractions protocols, each in combination with three different methods of cell lysis, and a commercial kit were evaluated. Criteria used for comparison of each method included the extracted protein concentration and the number of identified pr...
متن کاملEmpirical Evaluation of Bone Extraction Protocols
The application of high-resolution analytical techniques to characterize ancient bone proteins requires clean, efficient extraction to obtain high quality data. Here, we evaluated many different protocols from the literature on ostrich cortical bone and moa cortical bone to evaluate their yield and relative purity using the identification of antibody-antigen complexes on enzyme-linked immunosor...
متن کاملSoil and leaf litter metaproteomics—a brief guideline from sampling to understanding
The increasing application of soil metaproteomics is providing unprecedented, in-depth characterization of the composition and functionality of in situ microbial communities. Despite recent advances in high-resolution mass spectrometry, soil metaproteomics still suffers from a lack of effective and reproducible protein extraction protocols and standardized data analyses. This review discusses t...
متن کاملRNA isolation from soil for bacterial community and functional analysis: evaluation of different extraction and soil conservation protocols.
The impact of three different RNA isolation methods on the community analysis of metabolically active bacteria was determined by reverse transcription (RT) and PCR amplification of 16S rRNA genes and subsequent terminal restriction fragment length polymorphism (T-RFLP) analysis. Furthermore, soil samples were stored at different conditions in order to evaluate the effect of soil conservation me...
متن کاملAutomated Comparative Performance Evaluation of Security Protocols
We propose a comparative performance evaluation method for security protocols. We start by constructing a security protocol model where we assign a cost functions for each cryptographic operation. For each class of cryptographic operations (e.g. symmetric encryption, asymmetric decryption), we construct a polynomial function based on an exhaustive performance evaluation of cryptographic combina...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Soil Biology and Biochemistry
سال: 2012
ISSN: 0038-0717
DOI: 10.1016/j.soilbio.2012.05.014